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IGF1 ELISA Kit

Dieses Ratte IGF1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte IGF1 zu quantifizieren. Dieses ELISA Kit wurde in 12+ Publikationen zitiert.
Produktnummer ABIN411285

Kurzübersicht für IGF1 ELISA Kit (ABIN411285)

Target

Alle IGF1 ELISA Kits anzeigen
IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Bindungsspezifität

AA 49-118

Reaktivität

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Ratte

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5-4000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    62.5 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGF-1

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: G49-A118

    Kreuzreaktivität (Details)

    There is detectable cross-reactivity with IGF-2< 1 % .

    Homologie

    Hamster

    Sensitivität

    <5pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: G49-A118
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the insulin family.

    Plattentyp

    Pre-coated

    Protokoll

    rat IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been precoated onto 96-well plates. Standards(E.coli, G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-1 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGF-1 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 32.67, CV(%): 6.2
    • Sample 2: n=16, Mean(pg/ml): 1441, Standard deviation: 56.20, CV(%): 3.9
    • Sample 3: n=16, Mean(pg/ml): 2641, Standard deviation: 140, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 497, Standard deviation: 41.25, CV(%): 8.3
    • Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 82.73, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 2737, Standard deviation: 156, CV(%): 5.7

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

    Ozdemir, Aksu, Baykara, Ates, Sisman, Kiray, Buyuk, Uysal: "Effects of administration of subtoxic doses of acetaminophen on liver and blood levels of insulin-like growth factor-1 in rats." in: Toxicology and industrial health, Vol. 32, Issue 1, pp. 39-46, (2016) (PubMed).

    Cai, Tan, Zhang, Li, Wang, Zhu, Wang, Yang, Wang, Liu, Xu, Pan, Wang, Yang, Lu: "Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy." in: Stem cells translational medicine, Vol. 4, Issue 12, pp. 1425-35, (2016) (PubMed).

    Hou, Wan, Wang, Li, Wang, Yao, Feng, Jing, Lu, Jia, Peng: "Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression." in: American journal of translational research, Vol. 8, Issue 9, pp. 3656-3665, (2016) (PubMed).

    Niu, Kou, Zhou, Ding: "Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats." in: Neural regeneration research, Vol. 7, Issue 21, pp. 1611-7, (2015) (PubMed).

    Chen, Yang, He, Song, Liu: "Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis." in: Neural regeneration research, Vol. 8, Issue 19, pp. 1756-64, (2014) (PubMed).

    Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

    Cai, Li, Wang, Liu, Yang, Chen, Yin, Tan, Zhu, Pan, Wang, Lu: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." in: PLoS ONE, Vol. 8, Issue 5, pp. e63561, (2013) (PubMed).

    Aksu, Baykara, Kiray, Gurpinar, Sisman, Ekerbicer, Tas, Gokdemir-Yazar, Uysal: "Serum IGF-1 levels correlate negatively to liver damage in diabetic rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 3-4, pp. 194-201, (2013) (PubMed).

    Ni, Sun, Fu, Wang, Guo, Tian, Wei: "IGF-1 promotes the development and cytotoxic activity of human NK cells." in: Nature communications, Vol. 4, pp. 1479, (2013) (PubMed).

    Baykara, Aksu, Buyuk, Kiray, Sisman, Baykara, Dayi, Tas, Ozdemir, Arda, Uysal et al.: "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum ..." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 88, Issue 5, pp. 250-7, (2013) (PubMed).

    Iseli, Hedinger: "Histopathology and ultrastructure of primary adrenocortical nodular dysplasia with Cushing's syndrome." in: Histopathology, Vol. 9, Issue 11, pp. 1171-94, (1986) (PubMed).

  • Target Alle IGF1 ELISA Kits anzeigen

    IGF1 (Insulin-Like Growth Factor 1 (IGF1))

    Andere Bezeichnung

    IGF1

    Hintergrund

    Protein Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity). .

    Background: Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

    Synonyms: Insulin-like growth factor I,IGF-I,Somatomedin,Igf1,Igf-1,

    Full Gene Name: Insulin-like growth factor I

    Cellular Localisation: Secreted.

    Gen-ID

    24482

    UniProt

    P08025

    Pathways

    RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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