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CDKN1B ELISA Kit

CDKN1B Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN2748475
  • Target Alle CDKN1B ELISA Kits anzeigen
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Reaktivität
    • 7
    • 4
    • 4
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Mouse p27/Kip1 (CDKN1B) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Mouse p27/Kip1 (CDKN1B)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften CDKN1B ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CDKN1B ELISA Kits anzeigen
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Andere Bezeichnung
    p27/Kip1 (CDKN1B Produkte)
    Synonyme
    CDKN4 ELISA Kit, KIP1 ELISA Kit, MEN1B ELISA Kit, MEN4 ELISA Kit, P27KIP1 ELISA Kit, cdkn1b ELISA Kit, sb:cb611 ELISA Kit, wu:fb63g10 ELISA Kit, wu:fb64g10 ELISA Kit, wu:fe18e03 ELISA Kit, CDKN1B ELISA Kit, Kip1 ELISA Kit, p27 ELISA Kit, FAM14D ELISA Kit, ISG12 ELISA Kit, ISG12A ELISA Kit, P27 ELISA Kit, Cdki1b ELISA Kit, AA408329 ELISA Kit, AI843786 ELISA Kit, p27Kip1 ELISA Kit, cdkn1bl ELISA Kit, cyclin dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Bb ELISA Kit, interferon alpha inducible protein 27 ELISA Kit, cyclin-dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Ba ELISA Kit, CDKN1B ELISA Kit, cdkn1bb ELISA Kit, IFI27 ELISA Kit, Cdkn1b ELISA Kit, cdkn1ba ELISA Kit
    Hintergrund
    P27/Kip1 (CDKN1B)
    Gen-ID
    821314
    UniProt
    Q43735
    Pathways
    Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie
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