TLR1 ELISA Kit

Produktnummer ABIN2044675

Maus TLR1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus TLR1.

Kurzübersicht für TLR1 ELISA Kit (ABIN2044675)

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails TLR1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.108 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Toll Like Receptor 1 (TLR1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 1 (TLR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 1 (TLR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 1 (TLR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Toll Like Receptor 1 (TLR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TLR1

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Andere Bezeichnung: Toll Like Receptor 1 (TLR1)

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades