PDK1 ELISA Kit

Produktnummer ABIN2044369

Ratte PDK1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte PDK1.

Kurzübersicht für PDK1 ELISA Kit (ABIN2044369)

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails PDK1 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.58 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Andere Bezeichnung: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effekt