JAK3 ELISA Kit

Produktnummer ABIN2043827

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human JAK3.

Kurzübersicht für JAK3 ELISA Kit (ABIN2043827)

Target: JAK3 (Janus Kinase 3 (JAK3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails JAK3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.066 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Janus Kinase 3 (JAK3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 3 (JAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 3 (JAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 3 (JAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Janus Kinase 3 (JAK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response