IL1A ELISA Kit

Produktnummer ABIN2043671

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte IL1A.

Kurzübersicht für IL1A ELISA Kit (ABIN2043671)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 pg/mL

Applikation: ELISA

Produktdetails IL1A ELISA Kit

Untere Nachweisgrenze: 0.78 pg/mL

Sensitivität: < 0.21 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Sensitivity Interleukin 1 Alpha (IL1a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints