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RPS6KB1 ELISA Kit

RPS6KB1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN1572218
  • Target Alle RPS6KB1 ELISA Kits anzeigen
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Reaktivität
    • 13
    • 9
    • 9
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RPS6Kb1 in human tissue homogenates and other biological fluids.
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of this index.
    Kreuzreaktivität (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensitivität
    0.058 ng/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Testdurchführung

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Testpräzision
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handhabung
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Haltbarkeit
    12 months
  • Target Alle RPS6KB1 ELISA Kits anzeigen
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Andere Bezeichnung
    RPS6Kb1 (RPS6KB1 Produkte)
    Synonyme
    PS6K ELISA Kit, S6K ELISA Kit, S6K-beta-1 ELISA Kit, S6K1 ELISA Kit, STK14A ELISA Kit, p70 S6KA ELISA Kit, p70(S6K)-alpha ELISA Kit, p70-S6K ELISA Kit, p70-alpha ELISA Kit, 10539 ELISA Kit, 7084 ELISA Kit, CG10539 ELISA Kit, DS6K ELISA Kit, Dmel\\CG10539 ELISA Kit, Dp70S6k ELISA Kit, Dp70[s6k] ELISA Kit, Dp70s6k ELISA Kit, S6k ELISA Kit, dS6K ELISA Kit, dS6k ELISA Kit, dp70[S6k] ELISA Kit, dp70s6k ELISA Kit, dps6k ELISA Kit, ds6k ELISA Kit, fs(3)07084 ELISA Kit, l(3)07084 ELISA Kit, p70 S6K ELISA Kit, p70/S6K ELISA Kit, p70S6K ELISA Kit, p70[S6 kinase] ELISA Kit, p70[S6K] ELISA Kit, p70[S6k] ELISA Kit, p70[S6kinase] ELISA Kit, p70s6K ELISA Kit, s6k ELISA Kit, s6k11 ELISA Kit, 2610318I15Rik ELISA Kit, 4732464A07Rik ELISA Kit, 70kDa ELISA Kit, AA959758 ELISA Kit, AI256796 ELISA Kit, AI314060 ELISA Kit, p70/85s6k ELISA Kit, p70s6k ELISA Kit, p70s6k-A ELISA Kit, p70-s6k ELISA Kit, ps6k ELISA Kit, rps6kb1 ELISA Kit, rps6kb1-A ELISA Kit, s6K1 ELISA Kit, stk14a ELISA Kit, fc51h01 ELISA Kit, wu:fc51h01 ELISA Kit, zgc:55713 ELISA Kit, ribosomal protein S6 kinase B1 ELISA Kit, Ribosomal protein S6 kinase ELISA Kit, ribosomal protein S6 kinase, polypeptide 1 ELISA Kit, ribosomal protein S6 kinase B1 L homeolog ELISA Kit, ribosomal protein S6 kinase B1 S homeolog ELISA Kit, ribosomal protein S6 kinase b, polypeptide 1b ELISA Kit, RPS6KB1 ELISA Kit, S6k ELISA Kit, Rps6kb1 ELISA Kit, rps6kb1.L ELISA Kit, rps6kb1.S ELISA Kit, rps6kb1b ELISA Kit
    Hintergrund
    Alternative name: PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha, P70-S6 Kinase 1, 70 kDa ribosomal protein S6 kinase 1, Serine/threonine-protein kinase 14A
    Gen-ID
    6198
    UniProt
    P23443
    Pathways
    PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effekt
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