Catcher-Control

Produktnummer ABIN6953228

Kurzübersicht für Catcher-Control (ABIN6953228)

Applikation: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Protein Complex Immunoprecipitation (Co-IP)

Bead Matrix: Agarose beads

Produktdetails

Verwendungszweck: Catcher-Control is a control resin for all Catcher resins, which are based on 4 % crosslinked agarose beads.

Produktmerkmale: Catcher-Control is a control resin for all Catcher resins, which are based on 4 % cross-linked agarose beads. It was prepared using the same activation and quenching chemistry that was used for preparation of Catcher resins, but is not charged with any single-domain antibody.
Catcher-Control is thus the ideal resin for pre-depletion of extracts or as a side-by-side specificity control for any Catcher resins.
When using as a specificity control, Catcher-Control and the corresponding sdAb-charged Catcher Resin should be used under identical experimental conditions.

Anwendungsinformationen

Applikationshinweise: Coating: Same chemistry as for Catcher resins, but without immobilized single-domain antibody.
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Buffer Compatibility:

• Common buffer substances at pH 5 to 9
• 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
• 4 M urea
• 10 mM DTT, 10 mM 2-Mercaptoethanol
• RNAse A, DNAse I, Benzonase, protease inhibitors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 50 % slurry in PBS containing 20 % Ethanol

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4 °C, do not freeze

Haltbarkeit: 12 months