Protein A Resin

Details zu Produkt Nr. ABIN1536564, Anbieter: Anmelden zum Anzeigen
  • alpha protein
  • repB
Staphylococcus aureus (S. aureus)
Affinity Chromatography (AC), Purification (Purif)
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Verwendungszweck Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Produktmerkmale Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Ligand: Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand: 5
MW of ligand: Approximately 34 kDa
PI of ligand: 5.17
Degree of substitution: Approximately 2 mg protein A/ml settled resin
Static binding capacity: >20 mg rabbit IgG/ml settled resin
Matrix: spherical agarose, 4%
Average particle size: 90 μm (45-165 μm)
Bead Ligand Protein A
Bead Matrix Agarose beads
Bead Size 90 µm
Hintergrund Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens. 

High temperature heating is not recommended. The agarose melts above 65°C.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 1X PBS containing 20% ethanol
Lagerung 4 °C
Informationen zur Lagerung Store regenerated Protein A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
Haltbarkeit 18 months
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