Protein G Resin

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Affinity Chromatography (AC), Purification (Purif)
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Verwendungszweck Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. 
Produktmerkmale The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
- Number of IgG binding sites per ligand: 3
- MW of ligand: Approximately 22 kDa
- PI of ligand: 4.69
- Degree of substitution: Approximately 2 mg protein G/ml settled resin
- Static binding capacity: >20 mg sheep IgG/ml settled resin
- Matrix spherical: agarose, 4% cross-linked
- Average particle size: 90 μm (45-165 μm)
Bead Ligand Protein G
Bead Matrix Agarose beads
Bead Size 90 µm
Hintergrund Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Broad IgG binding spectrum
Binding specificity complements of protein A
Agarose media
No specific albumin binding
Optimized homogeneous recombinant ligand
High capacity

High temperature heating is not recommended. The agarose melts above 65°C.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 1X PBS containing 20% ethanol
Handhabung High temperature heating is not recommended. The agarose melts above 65°C.
Lagerung 4 °C
Informationen zur Lagerung Store regenerated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
Haltbarkeit 18 months
Produkt verwendet in: Tukaj, Grüner, Tukaj, Zillikens, Kasperkiewicz: "Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid." in: Journal of the European Academy of Dermatology and Venereology : JEADV, 2015 (PubMed).

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