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Monoklonale Antikörper

Monoklonale Antikörper sind Antikörper, die von identischen Immunzellen gebildet werden, die alle Klone einer eindeutigen Mutterzelle sind und an das gleiche Epitop binden. Bei fast jeder Substanz ist es möglich, monoklonale Antikörper herzustellen, die spezifisch an diese Substanz binden; sie können dann dazu dienen, diese Substanz aufzuspüren oder zu reinigen. Dies hat sich zu einem wichtigen Werkzeug in der Biochemie, Molekularbiologie und Medizin entwickelt. Unterscheiden Sie zwischen Wirt, Klon und Antigen, um Ihren gewünschten Antikörper in unserem Shop zu erhalten.

Produkt

Clone

Kat. Nr.

Menge

anti-TARDBP Antikörper (AA 1-260)

Clone 2E2-D3

Kat. Nr. ABIN565080

Menge 100 μg

anti-beta Amyloid Antikörper (C-Term)

Clone 9E8-1A3

Kat. Nr. ABIN5508784

Menge 100 μg

anti-ATM Antikörper (pSer1981)

Clone 10H11-E12

Kat. Nr. ABIN6656104

Menge 100 μg

anti-NFKB1 Antikörper (AA 860-969)

Clone 4A11

Kat. Nr. ABIN561981

Menge 100 μg

anti-SYN1 Antikörper (AA 362-511)

Clone 7H10G6

Kat. Nr. ABIN5542390

Menge 100 μL

Wir bieten Ihnen mehr als 100.000 hochwertige monoklonale Antikörper für eine Vielzahl von Anwendungen. Auf antibodies-online.com finden Sie zusätzlich eine Vielzahl von konjugierten monoklonalen Antikörpern mit Tags wie HA, HIS, Farbstoffen und den meisten anderen relevanten Konjugaten. Unser Support hilft Ihnen gerne weiter, wenn Sie weitere Fragen haben. Wenn Sie einen unkonjugierten Primärantikörper verwenden möchten, vergessen Sie nicht den passenden Sekundärantikörper!

Ausgewählte Targets

Cortisol Antibodies

Estrogen Receptor 1 Antibodies

BrDU Antibodies

HMGB1 Antibodies

ErbB2/Her2 Antibodies

Histone 4 Antibodies

Histone 3 Antibodies

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CALB1 Antikörper

CALB1 Reaktivität: Human WB, IHC, IF Wirt: Maus Monoclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin (CALB1) antibody (ABIN6254097)

Western Blotting validation image for anti-Calbindin (CALB1) antibody (ABIN6254097)

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.

Immunostaining of rat cerebellum showing specific labeling of calbindin (red) in the dendrites of Purkinje cells.

4 Abbildungen

Independent Validation

2 validations

Produktnummer ABIN6254097

Datenblatt (PDF)

Independently Validated

CLNS1A Antikörper

CLNS1A Reaktivität: Human WB, ICC, DB Wirt: Maus Monoclonal 2187C2a unconjugated

Western Blotting validation image for anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) antibody (ABIN933127)

Immunofluorescence validation image for anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) antibody (ABIN933127)

Western Blotting (WB) image for anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) antibody (ABIN933127)

Immunostaining analysis in HeLa cells.

4 Abbildungen

Independent Validation

2 validations

Produktnummer ABIN933127

Datenblatt (PDF)

Independently Validated

beta Actin Antikörper (AA 1-25)

ACTB Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh) WB, IHC (p) Wirt: Maus Monoclonal 1A2 unconjugated

DrFundc1 reduced cell viability while inducing autophagy and apoptosis in transgenic 293T cells.

Lane 1: 293T, Lane 2: NIH/3T3, Lane 3: mouse muscle lysates probed with beta-Actin (1A2) Monoclonal Antibody (bsm-33036M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Effect of rTMS on apoptosis and viability of neurons in hippocampus regions of AD mice.

Effect of rTMS on the activities of GSK-3β, Tau, and β-catenin in AD mice.

NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia.

NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia.

NgBR expression is downregulated in the pulmonary artery of HPH rat model and in vascular smooth muscle cells (VSMCs) exposed to hypoxia.

7 Abbildungen

1 reference

Produktnummer ABIN4906581

Datenblatt (PDF)

Cytokeratins Antikörper

Reaktivität: Säugetier ICFC, ICC, IHC (p), IP, WB Wirt: Maus Monoclonal C-11 unconjugated

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 ((ABIN94275), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green).

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

6 Abbildungen

6 Referenzen

Produktnummer ABIN94275

Datenblatt (PDF)

Integrin beta 2 Antikörper

ITGB2 Reaktivität: Human FACS, WB, IP, IHC (fro), Func, IHC (p) Wirt: Maus Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 Abbildungen

5 Referenzen

Produktnummer ABIN2749019

Datenblatt (PDF)

CD247 Antikörper (pTyr72)

CD247 Reaktivität: Human, Maus WB, ICC, ICFC Wirt: Maus Monoclonal EM-26 unconjugated

Anti-Hu CD3 zeta (pY72) Purified (clone EM-26) specificity verification by WB.

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants.

Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26, light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) works in Flow Cytometry application Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol.

Separation of pervanadate stimulated Jurkat cell suspension stained using anti-human CD3 zeta (pY72) (EM-26) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from pervanadate stimulated Jurkat cell suspension unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

6 Abbildungen

1 reference

Produktnummer ABIN2749060

Datenblatt (PDF)

Gelsolin Antikörper (AA 170-423)

GSN Reaktivität: Rind (Kuh) WB, IHC, IP, ICC Wirt: Maus Monoclonal C2 unconjugated

Detection of GSN in Bovine Lung lysate using Monoclonal Antibody to Gelsolin (GSN)

Detection of GSN in Bovine Cerebrum Tissue using Monoclonal Antibody to Gelsolin (GSN)

Detection of GSN in Bovine Kidney Tissue using Monoclonal Antibody to Gelsolin (GSN)

Detection of GSN in Bovine Cerebrum lysate using Monoclonal Antibody to Gelsolin (GSN)

Detection of GSN in Bovine Heart lysate using Monoclonal Antibody to Gelsolin (GSN)

Detection of GSN in Human A549 cell lysate using Monoclonal Antibody to Gelsolin (GSN)

6 Abbildungen

1 reference

Produktnummer ABIN7428656

Datenblatt (PDF)

Src Antikörper

Src Reaktivität: Human, Maus WB, IF Wirt: Maus Monoclonal 17AT28 unconjugated

Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody (ABIN387822 and ABIN2843908).

Anti-SRC Antibody at 1:500 dilution + HT-29 whole cell lysate Lysates/proteins at 20 μg per lane.

Fluorescent image of A549 cell stained with SRC Antibody (ABIN387822 and ABIN2843908)/SD41014A.

Anti-SRC Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 μg per lane.

The anti-Src Mab (ABIN387822 and ABIN2843908) is used in Western blot to detect Src in Jurkat cell lysate.

FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).

6 Abbildungen

3 Referenzen

Produktnummer ABIN387822

Datenblatt (PDF)

KRT8 Antikörper

KRT8 Reaktivität: Human, Maus WB, IHC, FACS, ICC, IHC (fro) Wirt: Maus Monoclonal M20 unconjugated

Immunohistochemistry on frozen section of human colon

Image no. 1 for anti-Keratin 8 (KRT8) antibody (ABIN335327)

Image no. 2 for anti-Keratin 8 (KRT8) antibody (ABIN335327)

Image no. 3 for anti-Keratin 8 (KRT8) antibody (ABIN335327)

Immunohistochemistry on frozen section of human colon

5 Abbildungen

10 Referenzen

Produktnummer ABIN335327

Datenblatt (PDF)

EZH2 Antikörper

EZH2 Reaktivität: Human WB, IF, IHC (p) Wirt: Maus Monoclonal 144CT2-1-1-5 unconjugated

All lanes : Anti-EZH2 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: T47D whole cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1 am1836a.

Fluorescent image of cell stained with EZH2 Antibody (ABIN659002 and ABIN2838040) / SG100830.

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.

EZH2 Monoclonal Antibody (ABIN659002 and ABIN2838040) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining.

A staining EZH2 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

6 Abbildungen

1 reference

Produktnummer ABIN659002

Datenblatt (PDF)

Syndecan 1 Antikörper (C-Term)

SDC1 Reaktivität: Human WB, FACS, IF Wirt: Maus Monoclonal 587CT7-3-6-5 unconjugated

C Antibody (C-term)(Ascites) ABIN1539995 western blot analysis in HepG2 cell line lysates (35 μg/lane).

C Antibody (C-term) (Ascites) ABIN1539995 flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).

Confocal immunofluorescent analysis of C Antibody (C-term) (Ascites) ABIN1539995 with cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

Confocal immunofluorescent analysis of C Antibody (C-term) (Ascites) ABIN1539995 with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

Confocal immunofluorescent analysis of C Antibody (C-term) (Ascites) ABIN1539995 with T47D cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).

C Antibody (C-term) (Ascites) ABIN1539995 flow cytometric analysis of cells (right histogram) compared to a negative control cell (left histogram).

C Antibody (C-term) (Ascites) ABIN1539995 flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).

7 Abbildungen

1 reference

Produktnummer ABIN1539995

Datenblatt (PDF)

CD44 Antikörper

CD44 Reaktivität: Human, Maus FACS, IHC, ELISA, ICC Wirt: Maus Monoclonal 8E2F3 unconjugated

Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).

Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization.

Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green).

Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.

Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.

Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).

7 Abbildungen

5 Referenzen

Produktnummer ABIN969026

Datenblatt (PDF)

FcRn Antikörper

FcRn Reaktivität: Human ELISA, IF, FACS, cELISA Wirt: Maus Monoclonal DVN24 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone DVN24 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE.

Tracer plasma concentrations were determined by ELISA and plotted either as percent remaining as compared with the first time point plasma concentrations or as plasma concentrations ±SD.

ELISA binding of human, macaque, pig, dog, mouse, and rat FcRn toward DVN24 at pH 7.

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone DVN24 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary.

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone DVN24 primary Ab and Texas Red™ conjugated anti-mouse secondary.

6 Abbildungen

6 Referenzen

Produktnummer ABIN1774763

Datenblatt (PDF)

SQSTM1 Antikörper (AA 232-356)

SQSTM1 Reaktivität: Human WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 5H7E2 unconjugated

Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).

Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein.

Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SQSTM1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.

7 Abbildungen

1 reference

Produktnummer ABIN1724836

Datenblatt (PDF)

NPC1 Antikörper (AA 34-174)

NPC1 Reaktivität: Human ELISA, IHC, FACS Wirt: Maus Monoclonal 8D10G3 unconjugated

Western blot analysis using NPC1 mAb against human NPC1 recombinant protein.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).

Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA: 34-174)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NPC1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NPC1 mouse mAb with DAB staining.

6 Abbildungen

2 Referenzen

Produktnummer ABIN1724840

Datenblatt (PDF)

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