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MAP2 Antikörper (Microtubule-Associated Protein 2) Primary Antibody

MAP2 Reaktivität: Human, Maus, Schwein ELISA, ICC, IHC (fro), IHC (p), IP, mIHC, WB Wirt: Maus Monoclonal MT-08 unconjugated
Pubmed (1 reference) Independent Validation (1 Validierung)
Produktnummer ABIN125739
$384.05
Zzgl. Versandkosten $45.00
0.1 mg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 5 bis 7 Werktagen
  • Target Alle MAP2 Antikörper anzeigen
    MAP2
    Reaktivität
    • 162
    • 107
    • 96
    • 31
    • 24
    • 7
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Schwein
    Wirt
    • 132
    • 47
    • 12
    • 2
    Maus
    Klonalität
    • 147
    • 45
    Monoklonal
    Konjugat
    • 96
    • 18
    • 15
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Dieser MAP2 Antikörper ist unkonjugiert
    Applikation
    • 115
    • 73
    • 59
    • 39
    • 39
    • 36
    • 32
    • 31
    • 22
    • 20
    • 18
    • 11
    • 4
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Multiplex Immunohistochemistry (mIHC), Western Blotting (WB)
    Spezifität
    The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).
    Kreuzreaktivität (Details)
    Human, Porcine, Mouse
    Aufreinigung
    Purified by protein-A affinity chromatography
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Microtubule protein (bovine brain) enriched for kinesin
    Klon
    MT-08
    Isotyp
    IgG1
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104333 (Multiplex Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Akoya Biosciences
    No.
    #104333
    Datum
    20.04.2021
    Antigen
    MAP2
    Chargennummer
    536958
    Validierte Anwendung
    Multiplex Immunohistochemistry
    Positivkontrolle

    Fresh frozen mouse olfactory bulb

    Negativkontrolle

    Unlabeled control (mouse fresh frozen)

    Bewertung

    Passed. The anti-MAP2 antibody ABIN125739 specifically labels structural elements of neurons. Staining can be observed in neuronal cell bodies, axons and occasionally in proximal dendritic processes.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN125739
    Sekundärantikörper
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-MAP2 antibody ABIN125739 to an oligo barcode used to bind oligo-bound fluorophore ATTO 550.
    Anmerkungen
    • No signal was detected in unlabeled specimens.

    • Specific staining of MAP2 was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Dzijak, Yildirim, Kahle, Novák, Hnilicová, Venit, Hozák: "Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner." in: PLoS ONE, Vol. 7, Issue 1, pp. e30529, (2012) (PubMed).

  • Target
    MAP2
    Andere Bezeichnung
    MAP2ab (MAP2 Produkte)
    Synonyme
    MAP2A, MAP2B, MAP2C, MAP2R, Mtap2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, zgc:103474, zgc:113974, microtubule associated protein 2, microtubule-associated protein 2, microtubule associated protein 2 S homeolog, MAP2, Map2, map2.S, map2
    Hintergrund
    MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
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