MAP2 Antikörper

Produktnummer ABIN361345

Produktdetails anti-MAP2 Antikörper

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser MAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Specific for the ~ 280k MAP2 protein.

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus)

Sensitivität: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.

Aufreinigung: Total IgY fraction

Immunogen: recombinant bovine MAP2 protein expressed in and purified from E. Coli

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:20,000 IF: 1:2,500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-MAP2 Antikörper (ABIN361345)

Woike, Wang, Tibbe, Hassani Nia, Failla, Kibæk, Overgård, Larsen, Fagerberg, Barsukov, Kreienkamp: "Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders." in: Scientific reports, Vol. 12, Issue 1, pp. 902, (2022) (PubMed).

Hassani Nia, Woike, Bento, Niebling, Tibbe, Schulz, Hirnet, Skiba, Hönck, Veith, Günther, Scholz, Bierhals, Driemeyer, Bend, Failla, Lohr, Alai, Kreienkamp: "Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans." in: Molecular psychiatry, (2022) (PubMed).

Hassani Nia, Woike, Martens, Klüssendorf, Hönck, Harder, Kreienkamp: "Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus." in: Molecular autism, Vol. 11, Issue 1, pp. 85, (2021) (PubMed).

Hassani Nia, Woike, Kloth, Kortüm, Kreienkamp: "Truncating mutations in SHANK3 associated with global developmental delay interfere with nuclear β-catenin signaling." in: Journal of neurochemistry, (2020) (PubMed).

Ho, Dallalzadeh, Karathanasis, Keles, Vangala, Grogan, Poirazi, Martin: "GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons." in: Molecular and cellular neurosciences, Vol. 61, pp. 1-12, (2014) (PubMed).

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Andere Bezeichnung: MAP2 (MAP2 Produkte)

Synonyme: MAP2A antikoerper, MAP2B antikoerper, MAP2C antikoerper, MAP2R antikoerper, Mtap2 antikoerper, MAP-2 antikoerper, Mtap-2 antikoerper, xmap2 antikoerper, MGC53131 antikoerper, a730034c02 antikoerper, zgc:103474 antikoerper, zgc:113974 antikoerper, microtubule associated protein 2 antikoerper, microtubule-associated protein 2 antikoerper, microtubule associated protein 2 S homeolog antikoerper, MAP2 antikoerper, Map2 antikoerper, map2.S antikoerper, map2 antikoerper

Hintergrund: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ( (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ( (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Anti-MAP2 Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Right:Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

Molekulargewicht: '280 kDa

Gen-ID: 4133

UniProt: P11137