ZAP70 Antikörper (Internal Region)

Produktnummer ABIN987274

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ZAP70 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ZAP70 Antikörper (Internal Region) (ABIN987274)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ZAP70

Sequenz: SDGYTPEPAR ITSPD

Spezifität: This antibody is expected to recognize isoform 1 (NP_001070.2) only.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (201

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70

Hintergrund: ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

Gen-ID: 7535, 22637, 301348

NCBI Accession: NP_001070

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway