CIITA Antikörper (AA 1-333)

Produktnummer ABIN97339

Hersteller Produkt- Nr.: 100-401-249

Produktdetails anti-CIITA Antikörper

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Bindungsspezifität: AA 1-333

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CIITA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 100-401-249

Hersteller: Rockland

Verwendungszweck: CIITA Antibody

Produktmerkmale: Synonyms: rabbit anti-CIITA Antibody, MHC class II transactivator, CIITA, MHC2TA

Aufreinigung: Anti-CIITA antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-CIITA antibody has been tested in western blot. For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CIITA Antikörper (ABIN97339)

Majumder, Lee, Rahmberg, Kumar, Mi, Scharer, Boss: "A super enhancer controls expression and chromatin architecture within the MHC class II locus." in: The Journal of experimental medicine, Vol. 217, Issue 2, (2020) (PubMed).

Scharer, Choi, Barwick, Majumder, Lohsen, Boss: "Genome-wide CIITA-binding profile identifies sequence preferences that dictate function versus recruitment." in: Nucleic acids research, Vol. 43, Issue 6, pp. 3128-42, (2015) (PubMed).

Koues, Dudley, Mehta, Greer: "The 19S proteasome positively regulates histone methylation at cytokine inducible genes." in: Biochimica et biophysica acta, Vol. 1789, Issue 11-12, pp. 691-701, (2009) (PubMed).

Details zu CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Andere Bezeichnung: CIITA (CIITA Produkte)

Hintergrund: Background: Anti-CIITA antibody detects CIITA. The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Gen-ID: 4261

NCBI Accession: NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints