Dynactin 1 Antikörper (AA 3-202)

Produktnummer ABIN967996

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Dynactin 1 in WB, IF, IP und IHC (zinc). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Huhn und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Dynactin 1 Antikörper (AA 3-202) (ABIN967996)

Target: Dynactin 1 (DCTN1)

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Klon: 1-p150Glued

Produktdetails anti-Dynactin 1 Antikörper

Bindungsspezifität: AA 3-202

Kreuzreaktivität: Human, Huhn, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p150 [Glued] aa. 3-202

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Dynactin 1 Antikörper (ABIN967996)

Kurzchalia: "Anthrax toxin rafts into cells." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 295-6, (2003) (PubMed).

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, (2002) (PubMed).

Aumais, Tunstead, McNeil, Schaar, McConnell, Lin, Clark, Yu-Lee: "NudC associates with Lis1 and the dynein motor at the leading pole of neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 24, pp. RC187, (2001) (PubMed).

Tai, Chuang, Sung: "Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1499-509, (2001) (PubMed).

King, Schroer: "Dynactin increases the processivity of the cytoplasmic dynein motor." in: Nature cell biology, Vol. 2, Issue 1, pp. 20-4, (2000) (PubMed).

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: p150 Glued

Hintergrund: P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 150 kDa

Pathways: M Phase, ER-Nucleus Signaling