ZAP70 Antikörper (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619)

Details for Product anti-ZAP70 Antibody No. ABIN967848, Anbieter: Anmelden zum Anzeigen
Antigen
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70
  • tyrosine-protein kinase ZAP-70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T-cell receptor associated protein kinase 70
  • zap70.L
  • zap70
  • ZAP70
  • LOC707555
  • Smp_149470
  • LOC100342021
  • LOC100390922
  • LOC100542877
  • Zap70
Epitop
AA 468-619
79
52
48
36
34
24
24
15
13
12
10
10
10
8
8
8
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Human, Maus, Ratte (Rattus)
581
217
138
14
11
10
8
8
6
6
4
3
1
Wirt
Maus
312
252
16
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser ZAP70 Antikörper ist unkonjugiert
33
26
22
19
13
11
9
9
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
322
185
179
163
158
82
52
50
22
7
5
5
4
4
4
3
3
2
2
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Human ZAP-70 Kinase aa. 468-619
Klon 29-ZAP70 Kinase
Isotyp IgG2a
Kreuzreaktivität Maus, Ratte (Rattus), Huhn
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung ZAP-70 (ZAP70 Antibody Abstract)
Hintergrund ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.
Molekulargewicht 70 kDa
Pathways T-Zell Rezeptor Signalweg
Kommentare

Related Products: ABIN968537, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.
Bilder des Herstellers
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 468-619) antibody (ABIN967848) Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia...
Produkt verwendet in: Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001 (PubMed).

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001 (PubMed).

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000 (PubMed).

Allgemeine Veröffentlichungen Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

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