ZAP70 Antikörper (AA 468-619)

Produktnummer ABIN967848

Dieses Anti-ZAP70-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ZAP70 in WB, IHC, IF und IP. Geeignet für Human, Maus, Ratte und Huhn. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für ZAP70 Antikörper (AA 468-619) (ABIN967848)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 29-ZAP70 Kinase

Produktdetails anti-ZAP70 Antikörper

Bindungsspezifität: AA 468-619

Kreuzreaktivität: Maus, Ratte (Rattus), Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ZAP-70 Kinase aa. 468-619

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ZAP70 Antikörper (ABIN967848)

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, (2001) (PubMed).

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, (2001) (PubMed).

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, (2000) (PubMed).

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP-70

Hintergrund: ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.

Molekulargewicht: 70 kDa

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway