ARAF Antikörper (AA 183-199)

Produktnummer ABIN967727

Der Maus Monoklonal Anti-ARAF-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, ARAF in Proben von Human, Maus, Ratte, Hund und Huhn zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für ARAF Antikörper (AA 183-199) (ABIN967727)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARAF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 1-A

Produktdetails anti-ARAF Antikörper

Bindungsspezifität: AA 183-199

Kreuzreaktivität: Human, Huhn, Hund, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse A-Raf aa. 183-199

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-ARAF Antikörper (ABIN967727)

Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, (2002) (PubMed).

Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, (1990) (PubMed).

Rapp, Heidecker, Huleihel, Cleveland, Choi, Pawson, Ihle, Anderson: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, (1989) (PubMed).

Huleihel, Goldsborough, Cleveland, Gunnell, Bonner, Rapp: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, (1987) (PubMed).

Details zu ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Andere Bezeichnung: A-Raf

Hintergrund: A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 68 kDa

Pathways: MAPK Signalweg, RTK Signalweg, Hepatitis C