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ARAF Antikörper

Name: ARAF Antikörper
SKU: ABIN5557283
Price: 490.77 EUR
Availability: InStock

ARAF Reaktivität: Human, Maus WB, IF (cc), IHC (p) Wirt: Kaninchen Monoclonal 24D1 unconjugated

HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody at 1:1000 overnight at 4˚C.

Paraformaldehyde-fixed, paraffin embedded human kidney section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with A-RAF (24D1) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

NIH/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

IF (cc)

HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

IF (cc)

4 Abbildungen

Produktnummer ABIN5557283

Datenblatt (PDF)

Target Alle ARAF Antikörper anzeigen

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
Reaktivität
127

51

33

4

4

4

3

3

2

2

1

1
Human, Maus
Wirt
121

6
Kaninchen
Passende Sekundärantikörper
Klonalität
112

15
Monoklonal
Konjugat
50

9

7

6

6

6

6

6

6

6

3

3

3

3

3

2

2
Dieser ARAF Antikörper ist unkonjugiert
Applikation
78

43

39

39

20

16

13

9

5

4

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität

Human, Maus

Aufreinigung

Purified by Protein A.

Immunogen

Recombinant human A-RAF protein, around 150-300aa.

Klon

24D1

Isotyp

IgG

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anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibody
ARAF Reaktivität: Human WB, IF, PLA Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody
ARAF Reaktivität: Human, Maus WB, IHC (p), IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody
ARAF Reaktivität: Human, Maus, Ratte, Hund, Huhn WB, IHC, IF, IP Wirt: Maus Monoclonal 1-A unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibody
ARAF Reaktivität: Human ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibody
ARAF Reaktivität: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibody
ARAF Reaktivität: Human, Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibody
ARAF Reaktivität: Human, Maus, Ratte WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 371-606) antibody
ARAF Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibody
ARAF Reaktivität: Human WB, IHC Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Andere Bezeichnung

A-RAF (ARAF Produkte)

Synonyme

zgc:92074 antikoerper, wu:fk45h07 antikoerper, ARAF antikoerper, a-raf antikoerper, araf antikoerper, araf1 antikoerper, pks2 antikoerper, rafa1 antikoerper, A-RAF antikoerper, ARAF1 antikoerper, PKS2 antikoerper, RAFA1 antikoerper, 1200013E08Rik antikoerper, A-Raf antikoerper, AW495444 antikoerper, Araf1 antikoerper, A-Raf proto-oncogene, serine/threonine kinase antikoerper, A-Raf proto-oncogene, serine/threonine kinase L homeolog antikoerper, A-Raf proto-oncogene, serine/threonine kinase S homeolog antikoerper, Araf proto-oncogene, serine/threonine kinase antikoerper, araf antikoerper, ARAF antikoerper, araf.L antikoerper, araf.S antikoerper, Araf antikoerper

Hintergrund

Synonyms: Serine/threonine-protein kinase A-Raf, ARAF, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF1, PKS, PKS2
Background: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Gen-ID

369

UniProt

P10398

Pathways

MAPK Signalweg, RTK Signalweg, Hepatitis C