ERK1 Antikörper (AA 325-345)

Produktnummer ABIN967701

Produktdetails anti-ERK1 Antikörper

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Bindungsspezifität: AA 325-345

Reaktivität: Human, Maus, Ratte, Huhn, Hund, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Human, Huhn, Hund, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat ERK1 aa. 325-345

Klon: MK12

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ERK1 Antikörper (ABIN967701)

Nowak: "Protein kinase C-alpha and ERK1/2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43377-88, (2002) (PubMed).

Aguirre-Ghiso, Liu, Mignatti, Kovalski, Ossowski: "Urokinase receptor and fibronectin regulate the ERK(MAPK) to p38(MAPK) activity ratios that determine carcinoma cell proliferation or dormancy in vivo." in: Molecular biology of the cell, Vol. 12, Issue 4, pp. 863-79, (2001) (PubMed).

Ackerley, Grierson, Brownlees, Thornhill, Anderton, Leigh, Shaw, Miller: "Glutamate slows axonal transport of neurofilaments in transfected neurons." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 165-76, (2000) (PubMed).

Wan, Huang: "Analysis of the Gs/mitogen-activated protein kinase pathway in mutant S49 cells." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14533-7, (1998) (PubMed).

Reszka, Seger, Diltz, Krebs, Fischer: "Association of mitogen-activated protein kinase with the microtubule cytoskeleton." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8881-5, (1995) (PubMed).

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: ERK1 (MAPK3 Produkte)

Hintergrund: The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is a 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kDa).

Molekulargewicht: 44 kDa

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine