Phospholipase C gamma 1 Antikörper (AA 82-100)

Produktnummer ABIN967699

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Phospholipase C gamma 1 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Huhn und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Phospholipase C gamma 1 Antikörper (AA 82-100) (ABIN967699)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phospholipase C gamma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 10-PLCgamma

Produktdetails anti-Phospholipase C gamma 1 Antikörper

Bindungsspezifität: AA 82-100

Kreuzreaktivität: Human, Maus, Ratte (Rattus), Hund, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow PLCgamma1 aa. 82-100

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-Phospholipase C gamma 1 Antikörper (ABIN967699)

Vossmeyer, Hofmann, Löster, Reutter, Danker: "Phospholipase Cgamma binds alpha1beta1 integrin and modulates alpha1beta1 integrin-specific adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4636-43, (2002) (PubMed).

Dayanir, Meyer, Lashkari, Rahimi: "Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17686-92, (2001) (PubMed).

Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, (2000) (PubMed).

Chen, Murphy-Ullrich, Wells: "A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility." in: The Journal of cell biology, Vol. 134, Issue 3, pp. 689-98, (1996) (PubMed).

Obermeier, Tinhofer, Grunicke, Ullrich: "Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation." in: The EMBO journal, Vol. 15, Issue 1, pp. 73-82, (1996) (PubMed).

Details zu Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Andere Bezeichnung: Phospholipase C gamma 1

Hintergrund: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCg activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCg1, regardless of phosphorylation status. It does not cross-react with PLCg2.

Molekulargewicht: 148 kDa

Pathways: RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling