PLK1 Antikörper (pThr210)

Produktnummer ABIN967616

Der Maus Monoklonal Anti-PLK1-Antikörper wurde für WB validiert. Er ist geeignet, PLK1 in Proben von Human und Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für PLK1 Antikörper (pThr210) (ABIN967616)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: K50-483

Produktdetails anti-PLK1 Antikörper

Bindungsspezifität: pThr210

Marke: BD Pharmingen™

Kreuzreaktivität (Details): Predicted: Mouse

Produktmerkmale: 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLK1 Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-PLK1 Antikörper (ABIN967616)

Peter, Gleixner, Cerny-Reiterer, Herrmann, Winter, Hadzijusufovic, Ferenc, Schuch, Mirkina, Horny, Pickl, Müllauer, Willmann, Valent: "Polo-like kinase-1 as a novel target in neoplastic mast cells: demonstration of growth-inhibitory effects of small interfering RNA and the Polo-like kinase-1 targeting drug BI 2536." in: Haematologica, Vol. 96, Issue 5, pp. 672-80, (2011) (PubMed).

Yamashiro, Yamakita, Totsukawa, Goto, Kaibuchi, Ito, Hartshorne, Matsumura: "Myosin phosphatase-targeting subunit 1 regulates mitosis by antagonizing polo-like kinase 1." in: Developmental cell, Vol. 14, Issue 5, pp. 787-97, (2008) (PubMed).

Ando, Ozaki, Yamamoto, Furuya, Hosoda, Hayashi, Fukuzawa, Nakagawara: "Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 24, pp. 25549-61, (2004) (PubMed).

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund: Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells.
The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Molekulargewicht: 68 kDa

Pathways: Zellzyklus, M Phase