BAX Antikörper (N-Term)

Produktnummer ABIN967537

Dieses Maus Monoklonal-Antikörper erkennt spezifisch BAX in WB und IP. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für BAX Antikörper (N-Term) (ABIN967537)

Target: BAX (BCL2-Associated X Protein (BAX))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BAX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 6A7

Produktdetails anti-BAX Antikörper

Bindungsspezifität: N-Term

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: N-terminal peptide sequence

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation. Daudi B lymphoma cells (ATCC CCL-213) and rat thymocytes are suggested as positive controls for western blot analysis. Due to low level of Bax expression in Hela cells, we do not recommend Hela as a positive control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-BAX Antikörper (ABIN967537)

Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, (1997) (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, (1997) (PubMed).

Krajewski, Krajewska, Shabaik, Miyashita, Wang, Reed: "Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2." in: The American journal of pathology, Vol. 145, Issue 6, pp. 1323-36, (1995) (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, (1994) (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, (1994) (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, (1993) (PubMed).

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: Bax

Hintergrund: The Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax may form homodimers or heterodimers with either Bcl-2 and Bcl-XL (long). Bax homodimers is thought to promote cell death, whereas Bax heterodimerization with either Bcl-2 and Bcl-XL appears to block cell death. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Bax expression has been identified in a variety of tissues, including lung, stomach, kidney, thymus, lymph nodes, bone marrow, spleen, heart, exocrine pancreas, and brain. Liver, kidney, and the exocrine pancreas have been found to contain little or no Bcl-2. The Bax gene potentially encodes three different proteins Bax-alpha (21 kDa), Bax-beta (24 kDa) and Bax-gamma (5 kDa). Bax-alpha is the most common transcript. The 6A7 antibody recognizes human, mouse and rat Bax. The 6A7 antibody reacts with an epitope between amino acids 12-24 of Bax, and studies suggest that this epitope may be in the vicinity of the dimerization domain of Bax. It has been proposed that the 6A7 antibody may directly compete with either Bcl-2 or Bcl-XL for binding to Bax. In western blots, the 6A7 antibody typically detects Bax as a single band of 21-22 kDa, thought to be Bax-alpha.

Molekulargewicht: 21-22 kDa

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response