ELK1 Antikörper

Produktnummer ABIN966064

Dieser Monoklonal Antikörper detektiert spezifisch ELK1 in WB, IHC und ELISA. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für ELK1 Antikörper (ABIN966064)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reaktivität: Human

Wirt: Bitte anfragen

Klonalität: Monoklonal

Konjugat: Dieser ELK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-ELK1 Antikörper

Spezifität: Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3).

Aufreinigung: Ascites.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500- 1: 1,000
IHC(P): 1: 500- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: -20 °C

Referenzen für anti-ELK1 Antikörper (ABIN966064)

Hsieh, Wu, Tsai, Huang, Hsieh, Liu: "PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells." in: Biochemical and biophysical research communications, Vol. 339, Issue 1, pp. 217-25, (2005) (PubMed).

Gille, Strahl, Shaw: "Activation of ternary complex factor Elk-1 by stress-activated protein kinases." in: Current biology : CB, Vol. 5, Issue 10, pp. 1191-200, (1996) (PubMed).

Gille, Kortenjann, Thomae, Moomaw, Slaughter, Cobb, Shaw: "ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation." in: The EMBO journal, Vol. 14, Issue 5, pp. 951-62, (1995) (PubMed).

Rao, Huebner, Isobe, ar-Rushdi, Croce, Reddy: "elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints." in: Science (New York, N.Y.), Vol. 244, Issue 4900, pp. 66-70, (1989) (PubMed).

Details zu ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Andere Bezeichnung: ELK1

Hintergrund: The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Gen-ID: 2002

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling