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CD25 Antikörper

Name: CD25 Antikörper
Brand: antibodies-online
SKU: ABIN965780
Price: 363 EUR
Availability: InStock

IL2RA Reaktivität: Human FACS, IHC (fro), IHC (af) Wirt: Maus Monoclonal B489 (143-13) unconjugated

(3 Referenzen)

Produktnummer ABIN965780

Datenblatt (PDF)

Target Alle CD25 (IL2RA) Antikörper anzeigen

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reaktivität
184

137

40

10

10

5

3

3

2

2

1

1
Human
Wirt
159

103

80

2

2

1

1
Maus
Passende Sekundärantikörper
Klonalität
272

70

4
Monoklonal
Konjugat
128

36

32

26

24

13

8

7

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD25 Antikörper ist unkonjugiert
Applikation
247

92

60

59

52

44

26

19

15

13

10

9

9

8

6

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

Spezifität

This antibody reacts a 55kD single chain glycoprotein, CD25, that associates with CD12 and CD132 to form the IL-2 receptor complex. CD25 has three epitope regions - A, B, and C - the latter of which is recognized by B489. This MAb has been clustered at the Fourth International Workshop on human differentiation antigens.

Aufreinigung

Concentrated.

Immunogen

Human leucocytes.

Klon

B489 (143-13)

Isotyp

IgG1

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Applikationshinweise

Immunohistochemistry (acetone fixed frozen tissue): 1:50-1:100
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue sections (acetone fixed). The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Kommentare

Recommended Positive Control: Tonsil, Thymus, Spleen

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.3 mg/ml.

Buffer

20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C
Parke: "Intravenous gammaglobulin in pregnancy, the Connecticut experience." in: Scandinavian journal of rheumatology. Supplement, Vol. 107, pp. 103-4, (1998) (PubMed).

Goldstein, Marcon, Cullen, Nelson: "A competitive enzyme-linked immunoassay (ELISA) for the measurement of soluble human interleukin-2 receptors (IL-2R, Tac protein)." in: Journal of immunological methods, Vol. 107, Issue 1, pp. 103-9, (1988) (PubMed).

Uchiyama, Nelson, Fleisher, Waldmann: "A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 4, pp. 1398-403, (1981) (PubMed).
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung

CD25 (IL-2R) (IL2RA Produkte)

Gen-ID

3559

Pathways

JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation