Src Antikörper (pTyr215) (AbBy Fluor® 647)

Produktnummer ABIN879252

Dieses Anti-Src-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Src in IF (cc) und IF (p). Geeignet für .

Kurzübersicht für Src Antikörper (pTyr215) (AbBy Fluor® 647) (ABIN879252)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Src Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-Src Antikörper (AbBy Fluor® 647)

Bindungsspezifität: pTyr215

Homologie: Human,Mouse,Rat,Dog,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse Src around the phosphorylation site of Tyr215

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Andere Bezeichnung: Src

Hintergrund:

Synonyms: SRCphospho Y215, ASV, Avian sarcoma virus, c SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, cSrc, EC 2.7.10.2, Neuronal CSRC tyrosine specic protein kinase, Neuronal SRC, Oncogene SRC, OTTHUMP00000030931, OTTHUMP00000174476, OTTHUMP00000174477, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, protooncogene SRC Rous sarcoma, Src, SRC Oncogene, SRC_HUMAN, SRC1, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog.

Background: Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. The tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of tyrosine 418 at the catalytic domain, thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated and Src becomes active. Src is a proto oncogene that may play a role in the regulation of embryonic development and cell growth. Mutations in this gene could be involved in the malignant progression of colon cancer. Immunogen: Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, based on the human sequence. The sequence is conserved in mouse (tyrosine 534), chicken (tyrosine 527) and frog (tyrosine 525).

Gen-ID: 6714

Pathways: JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling