Leptin Antikörper

Produktnummer ABIN799626

Der Kaninchen Polyklonal anti-Leptin Antikörper (ABIN799626) detektiert spezifisch Leptin in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Leptin Antikörper (ABIN799626)

Target: Leptin (LEP)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Leptin Antikörper

Spezifität: Recombinant mouse leptin 16000 MW produced in E. coli.

Aufreinigung: Protein G purified

Immunogen: Recombinant mouse LEP / Leptin.

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2400), WB (1:200 - 1:400)

Usage: This IgG fraction of anti-Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2400 is suggested for this product.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

Gen-ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process