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Leptin Antikörper (AA 101-154)

Name: Leptin Antikörper (AA 101-154)
SKU: ABIN725030
Price: 384.62 EUR
Availability: InStock

LEP Reaktivität: Maus, Ratte ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

3 Abbildungen

Produktnummer ABIN725030

Datenblatt (PDF)

Target Alle Leptin (LEP) Antikörper anzeigen

Leptin (LEP)
Bindungsspezifität
25

15

15

15

12

11

10

7

5

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-154
Reaktivität
127

74

72

12

8

7

5

3

3

3

2

1

1

1
Maus, Ratte
Wirt
178

24

6

5

2
Kaninchen
Passende Sekundärantikörper
Klonalität
189

25
Polyklonal
Konjugat
107

26

17

9

8

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

1
Dieser Leptin Antikörper ist unkonjugiert
Applikation
136

84

67

38

38

37

32

30

25

21

15

11

10

6

5

2

2

1

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Maus, Ratte

Homologie

Dog,Cow,Pig,Horse

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Leptin

Isotyp

IgG

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Applikationshinweise

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

Leptin (LEP)

Andere Bezeichnung

Leptin (LEP Produkte)

Synonyme

ob antikoerper, obese antikoerper, LEPD antikoerper, OB antikoerper, OBS antikoerper, leptin antikoerper, Lep antikoerper, LEP antikoerper, lep antikoerper

Hintergrund

Synonyms: ob, obese, Leptin, Obesity factor, Lep
Background: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Gen-ID

16846

UniProt

P41160

Pathways

JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process