IFNAR2 Antikörper (Carboxyfluorescein (CFS))

Produktnummer ABIN796985

Der Ziege Polyklonal Anti-IFNAR2-Antikörper wurde für FACS validiert. Er ist geeignet, IFNAR2 in Proben von Maus zu detektieren.

Kurzübersicht für IFNAR2 Antikörper (Carboxyfluorescein (CFS)) (ABIN796985)

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFNAR2 Antikörper ist konjugiert mit Carboxyfluorescein (CFS)

Applikation: Flow Cytometry (FACS)

Produktdetails anti-IFNAR2 Antikörper (Carboxyfluorescein (CFS))

Spezifität: Mouse IFNA/B R2.

Aufreinigung: Immunoaffinity purified

Immunogen: Mouse myeloma cell line NS0- derived recombinant human IFN-a/b R2 extracellular domain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: Flo

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Testdurchführung:

Flow Cytometry Validation
This antibody has been tested for flow cytometry using A20 cells.

1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
   
2. After blocking, 10ul of conjugated antibody was added to 1 - 2.5 x 10e5 cells and incubated for 30 minutes at room temperature.
   
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
   
4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
   
5. Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, up to 0.5 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles and prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for up to 1 year.

Haltbarkeit: 12 months

Details zu IFNAR2

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Andere Bezeichnung: IFNAR2

Hintergrund: Name/Gene ID: IFNAR2
Family: Interferon Receptor

Synonyms: IFNAR2, IFN-alpha/beta receptor 2, IFN-alpha-REC, IFN-alpha binding protein, IFN-R, IFN-R-2, IFNARB, Type I interferon receptor 2, IFNABR

Gen-ID: 3455

UniProt: P48551

Pathways: JAK-STAT Signalweg, Hepatitis C