Caspase 3 Antikörper (Internal Region)

Produktnummer ABIN768625

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Caspase 3 in WB, ELISA, ICC und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Caspase 3 Antikörper (Internal Region) (ABIN768625)

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Caspase 3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Caspase 3

Sequenz: RDVSKEDHSK RS

Spezifität: This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2, NP_116786.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 32 kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6 kDa according to Human NP_004337.2 and 31.4 kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65 kDa was also

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Caspase 3 Antikörper (ABIN768625)

Zhào, Liu, Zeng, Li, Zhang, Huang: "Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 9859672, (2018) (PubMed).

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: CASP3

Hintergrund: CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3

Molekulargewicht: 31.6kDa according to NP_004337.2

Gen-ID: 836

NCBI Accession: NP_004337, NP_116786

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation