MCM5 Antikörper (AA 500-734)

Produktnummer ABIN8093321

Der Maus Monoklonal anti-MCM5 Antikörper wird verwendet zum Nachweis von MCM5 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für MCM5 Antikörper (AA 500-734) (ABIN8093321)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Klon: MCM5-3200

Produktdetails anti-MCM5 Antikörper

Bindungsspezifität: AA 500-734

Verwendungszweck: MCM5 Antibody / Minichromosome maintenance complex component 5 (azide and preservative free)

Aufreinigung: Protein A affinity

Immunogen: A recombinant fragment (around amino acids 500-734) of human MCM5 protein (exact sequence is proprietary) was used as the immunogen for the MCM5/MCM complex protein 5 antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the MCM5/MCM complex protein 5 antibody should be determined by the researcher.

2. This MCM5/MCM complex protein 5 antibody is recombinantly produced by expression in CHO cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: MCM5/MCM complex protein 5 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Andere Bezeichnung: MCM5

Hintergrund: MCM5 Antibody recognizes MCM complex protein 5, a core component of the minichromosome maintenance helicase complex encoded by the MCM5 gene. MCM5 Antibody, also referred to as Minichromosome maintenance complex component 5 antibody and MCM complex protein 5 antibody in the literature, detects a nuclear replication factor that plays a central role in origin licensing and replication fork activation. Unlike markers that simply label cycling cells, MCM complex protein 5 is directly involved in assembling the pre-replication complex and preparing chromatin for DNA synthesis.

MCM complex protein 5 functions as part of the heterohexameric MCM2-7 complex, which forms the core replicative helicase responsible for unwinding double-stranded DNA during S phase. In early G1, MCM proteins are recruited to replication origins by ORC, CDC6, and CDT1, creating licensed origins poised for activation. Upon entry into S phase, phosphorylation events promote helicase activation, allowing replication fork progression. Because MCM5 remains chromatin-associated in cells that are replication-competent, MCM5 Antibody provides insight into replication readiness rather than only active DNA synthesis.

Subcellularly, MCM complex protein 5 localizes to the nucleus in proliferating epithelial, stromal, and hematopoietic cells. Its expression is low or absent in terminally differentiated or quiescent cells that have exited the cell cycle. This differential expression pattern makes MCM5 Antibody valuable for distinguishing cycling tumor cells from non-proliferative background tissue in immunohistochemical applications. Increased nuclear staining is commonly observed in colorectal carcinoma, breast carcinoma, lung adenocarcinoma, urothelial carcinoma, and high-grade dysplastic lesions, reflecting expanded replication licensing activity in malignant tissues.

Beyond its diagnostic relevance, dysregulation of MCM complex protein 5 contributes to replication stress and genomic instability. Overexpression can reflect unscheduled origin firing, while insufficient licensing may result in stalled forks and DNA damage signaling. MCM5 Antibody (clone MCM5/3200) is designed to detect endogenous MCM complex protein 5 in research applications, producing distinct nuclear staining patterns in actively cycling cells. By targeting a helicase subunit integral to origin activation, this antibody supports mechanistic studies of replication dynamics, chromatin loading of helicase complexes, and cell cycle checkpoint regulation.

Through evaluation of replication licensing activity and helicase assembly, MCM5 Antibody serves as a powerful tool for investigating cell cycle control, tumor proliferation biology, and DNA replication fidelity in both normal and neoplastic tissues.

UniProt: P33992

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA