CHEK1 Antikörper (pSer317)

Produktnummer ABIN757072

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CHEK1 in ELISA, IHC (p), ICC, IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus und Huhn und wurde in 1 Publikation erwähnt.

Kurzübersicht für CHEK1 Antikörper (pSer317) (ABIN757072)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Maus, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: pSer317

Spezifität: This phosphorylation site is homologous to that of Ser317 in Mouse and Rat.

Kreuzreaktivität: Huhn, Maus

Homologie: Human,Rat,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CHEK1 Antikörper (ABIN757072)

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHEK1

Hintergrund:

Synonyms: CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1

Background: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA.

Gen-ID: 1111

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur