WEE1 Antikörper (pSer123) (HRP)

Produktnummer ABIN756601

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch WEE1 in WB, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für WEE1 Antikörper (pSer123) (HRP) (ABIN756601)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WEE1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-WEE1 Antikörper (HRP)

Bindungsspezifität: pSer123

Verwendungszweck: Phospho-Wee1 (Ser123) Polyclonal Antibody, HRP Conjugated

Spezifität: This phosphorylation site is homologous to Ser123 in Mouse, Rat, and Bovine. The peptide used to produce this antibody has high homology with a sequence occurring in MIER3, and may react with this protein in Mouse and Human when phosphorylated at the site of Ser10.

Homologie: Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000),

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Andere Bezeichnung: Wee1

Hintergrund:

Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gen-ID: 7465

UniProt: P30291

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase