GLI2 Antikörper (AA 721-1457)

Produktnummer ABIN7993234

Der Kaninchen Polyklonal anti-GLI2 Antikörper wird verwendet zum Nachweis von GLI2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für GLI2 Antikörper (AA 721-1457) (ABIN7993234)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Produktdetails anti-GLI2 Antikörper

Bindungsspezifität: AA 721-1457

Verwendungszweck: Anti-Zinc finger protein GLI2 GLI2 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Zinc finger protein GLI2 GLI2 Antibody-5. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GLI2 recombinant protein (Position: K721-D1457).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: GLI2

Hintergrund:

Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Gene Full Name: GLI family zinc finger 2

Molekulargewicht: 210 kDa

Gen-ID: 2736

UniProt: P10070

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis