Caspase 3 Antikörper (AA 11-120)

Produktnummer ABIN747728

Der Kaninchen Polyklonal Anti-Caspase 3-Antikörper wurde für WB, ELISA, IHC (p), FACS, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, Caspase 3 in Proben von Human, Maus und Ratte zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Caspase 3 Antikörper (AA 11-120) (ABIN747728)

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Caspase 3 Antikörper

Bindungsspezifität: AA 11-120

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Caspase 3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Caspase 3 Antikörper (ABIN747728)

Duan, Li, Li, Xing, Zhang, Li, Zhao, Sun, Gao, Li et al.: "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic ..." in: Free radical biology & medicine, Vol. 94, pp. 74-87, (2016) (PubMed).

Zhang, Yu: "Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression." in: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 2, pp. 491-9, (2016) (PubMed).

Luo, Chen, Zhang, Chen, Li: "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 98, pp. 9-19, (2015) (PubMed).

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase 3

Hintergrund:

Synonyms: Lice, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, IRP, Protein Yama, SREBP cleavage activity 1, SCA-1, Casp3, Cpp32

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).

Gen-ID: 25402

UniProt: P55213

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation