WEE1 Antikörper (pSer642)

Produktnummer ABIN746873

Der Kaninchen-Polyklonal Anti-WEE1-Antikörper wurde für WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro) validiert. Er ist geeignet zum Nachweis von WEE1 in Proben aus Human. }

Kurzübersicht für WEE1 Antikörper (pSer642) (ABIN746873)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WEE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-WEE1 Antikörper

Bindungsspezifität: pSer642

Homologie: Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser642 [SV(p-S)LT]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Andere Bezeichnung: Wee1

Hintergrund:

Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gen-ID: 7465

UniProt: P30291

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase