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PRAS40 Antikörper (pThr246)

Der Kaninchen Polyklonal Anti-PRAS40-Antikörper wurde für WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, PRAS40 in Proben von Human zu detektieren.
Produktnummer ABIN744953

Kurzübersicht für PRAS40 Antikörper (pThr246) (ABIN744953)

Target

Alle PRAS40 (AKT1S1) Antikörper anzeigen
PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reaktivität

  • 77
  • 29
  • 21
  • 6
  • 5
  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 68
  • 11
Kaninchen

Klonalität

  • 61
  • 18
Polyklonal

Konjugat

  • 43
  • 6
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser PRAS40 Antikörper ist unkonjugiert

Applikation

  • 65
  • 39
  • 27
  • 13
  • 13
  • 11
  • 8
  • 4
  • 3
  • 3
  • 2
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 15
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr246

    Spezifität

    This phosphorylation site is homologous to Thr247 in Mouse and Rat.

    Homologie

    Human,Mouse,Rat,Dog,Cow,Horse

    Aufreinigung

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Thr246

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Target

    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

    Andere Bezeichnung

    PRAS40

    Hintergrund

    Synonyms: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1

    Background: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

    Gen-ID

    84335

    UniProt

    Q96B36

    Pathways

    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Cell Size, Autophagie, BCR Signaling, Warburg Effekt
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