Rekombinanter RNF7 Antikörper

Produktnummer ABIN7882181

Der Kaninchen Monoklonal anti-RNF7 Antikörper (Klon 30R91) (ABIN7882181) detektiert spezifisch RNF7 in WB, IHC, IF und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Rekombinanter RNF7 Antikörper (ABIN7882181)

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RNF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 30R91

Produktdetails anti-RNF7 Antikörper

Verwendungszweck: RNF7 Antibody / Ring finger protein 7

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human RNF7 was used as the immunogen for the RNF7 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the RNF7 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the RNF7 antibody at -20oC.

Details zu RNF7

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Andere Bezeichnung: RNF7

Hintergrund: RNF7 antibody detects Ring finger protein 7, encoded by the RNF7 gene. Ring finger protein 7 is a small RING type E3 ubiquitin ligase that is an essential component of the SCF (SKP1 CUL1 F box) ubiquitin ligase complex. This complex targets key regulatory proteins for ubiquitin mediated degradation, ensuring proper cell cycle progression, signal transduction, and stress responses. RNF7 antibody allows researchers to study the regulation of protein turnover and the molecular machinery that controls cellular homeostasis through ubiquitination.

Ring finger protein 7 has multiple cellular roles, including redox regulation, neddylation, and tumor suppressor control. By facilitating ubiquitination, RNF7 ensures timely degradation of proteins such as p27 and IkappaB, which govern cell cycle checkpoints and NF kappa B signaling respectively. Studies using RNF7 antibody have shown that disruption of this E3 ligase leads to aberrant accumulation of these regulators, causing uncontrolled proliferation or impaired immune responses. RNF7 also interacts with antioxidant pathways by mediating degradation of pro oxidant proteins, supporting redox balance in the cell.

Altered expression of Ring finger protein 7 has been implicated in cancer and degenerative diseases. Overexpression of RNF7 has been reported in several human cancers, including prostate, pancreatic, and lung carcinoma, where it may promote tumorigenesis by reducing levels of pro apoptotic proteins. Conversely, loss of function mutations impair ubiquitin ligase activity, leading to genomic instability. RNF7 antibody has been applied to studies that link this factor to chemotherapy resistance and cancer progression, making it an attractive target for drug discovery.

RNF7 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting detects expression in cancer cell lines, while immunohistochemistry demonstrates its nuclear and cytoplasmic distribution in tissue sections. Immunoprecipitation with RNF7 antibody enables isolation of the SCF complex for functional studies. Together, these applications allow researchers to investigate its role in proteostasis, stress response, and tumor biology.

By providing validated RNF7 antibody reagents, NSJ Bioreagents supports research into ubiquitin ligase function, cancer mechanisms, and redox biology, ensuring accurate detection of Ring finger protein 7 across diverse experimental systems.

UniProt: Q9UBF6

Pathways: Positive Regulation of Endopeptidase Activity