EVC2 Antikörper (AA 379-901)

Produktnummer ABIN7874490

Der Kaninchen Polyklonal anti-EVC2 Antikörper wird verwendet zum Nachweis von EVC2 in Proben von Maus und Ratte. Er wurde validiert für ELISA, IHC, WB und IF.

Kurzübersicht für EVC2 Antikörper (AA 379-901) (ABIN7874490)

Target: EVC2 (Ellis Van Creveld Syndrome 2 (EVC2))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EVC2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-EVC2 Antikörper

Bindungsspezifität: AA 379-901

Verwendungszweck: EVC2 Antibody / Ellis-van Creveld syndrome protein 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived mouse EVC2 recombinant protein (Position: Q379-D901) was used as the immunogen for the EVC2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the EVC2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the EVC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu EVC2

Target: EVC2 (Ellis Van Creveld Syndrome 2 (EVC2))

Andere Bezeichnung: EVC2

Hintergrund: EVC2 antibody detects Ellis-van Creveld syndrome protein 2, a ciliary basal body protein required for Hedgehog signaling and skeletal development. The UniProt recommended name is Ellis-van Creveld syndrome protein 2 (EVC2). Also known as Limbin, this protein anchors signaling complexes at the base of the primary cilium, coordinating receptor localization and developmental signal transduction.

Functionally, EVC2 antibody identifies a 1,285-amino-acid transmembrane protein localized to the ciliary pocket region. EVC2 forms a complex with EVC, tethering Hedgehog pathway components such as Smoothened and Gli transcription factors at the cilium base to modulate downstream transcriptional activity. It is essential for skeletal morphogenesis and growth plate function.

The EVC2 gene is located on chromosome 4p16.2 and is expressed in ciliated tissues, including cartilage, bone, and embryonic organs. Proper EVC2 function maintains ciliary integrity and ensures controlled Hedgehog pathway activation during tissue differentiation.

Pathologically, mutations in EVC2 cause Ellis-van Creveld syndrome and Weyers acrofacial dysostosis, characterized by short stature, polydactyly, and craniofacial abnormalities. Disruption of EVC2 impairs Hedgehog signaling, leading to developmental malformations. Research using EVC2 antibody supports studies in ciliopathies, developmental biology, and skeletal genetics.

EVC2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary and developmental proteins. NSJ Bioreagents provides EVC2 antibody reagents optimized for cilia structure, signaling, and morphogenesis research.

Structurally, Ellis-van Creveld syndrome protein 2 is a membrane-tethered scaffold with coiled-coil domains that mediate interaction with EVC and ciliary membrane components. This antibody enables examination of EVC2's function in Hedgehog signaling and developmental regulation.

UniProt: Q8K1G2

Pathways: Hedgehog Signalweg