SMPD3 Antikörper (AA 184-553)

Produktnummer ABIN7871479

Der Kaninchen Polyklonal anti-SMPD3 Antikörper (ABIN7871479) detektiert spezifisch SMPD3 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SMPD3 Antikörper (AA 184-553) (ABIN7871479)

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMPD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SMPD3 Antikörper

Bindungsspezifität: AA 184-553

Verwendungszweck: SMPD3 Antibody / Sphingomyelin phosphodiesterase 3 / nSMase2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SMPD3 recombinant protein (Position: F184-D553) was used as the immunogen for the SMPD3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SMPD3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SMPD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Andere Bezeichnung: SMPD3

Hintergrund: SMPD3 antibody detects Sphingomyelin phosphodiesterase 3, a membrane-associated enzyme that hydrolyzes sphingomyelin to generate ceramide and phosphocholine. The UniProt recommended name is Sphingomyelin phosphodiesterase 3 (SMPD3). This enzyme, also known as neutral sphingomyelinase 2 (nSMase2), plays a key role in lipid metabolism, cell signaling, and exosome biogenesis.

Functionally, SMPD3 antibody identifies a 655-amino-acid enzyme localized to the plasma membrane and Golgi apparatus. SMPD3 catalyzes the production of ceramide, a lipid second messenger involved in apoptosis, differentiation, and inflammation. It regulates membrane curvature and vesicle budding, particularly in exosome secretion and cell stress responses.

The SMPD3 gene is located on chromosome 16p13.12 and is expressed in brain, liver, and skeletal tissue. SMPD3 activity influences cell growth, neuronal communication, and lipid raft organization. By controlling ceramide levels, SMPD3 links sphingolipid metabolism to cell fate decisions and inflammatory signaling.

Pathologically, dysregulation of SMPD3 contributes to metabolic, neurodegenerative, and cardiovascular diseases. Deficiency results in skeletal abnormalities (spondyloepimetaphyseal dysplasia) and altered lipid homeostasis. Overactivation can enhance ceramide-induced apoptosis or inflammatory cascades. Research using SMPD3 antibody supports studies in lipid signaling, neurobiology, and exosome biology.

SMPD3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect sphingomyelinases and lipid signaling enzymes. NSJ Bioreagents provides SMPD3 antibody reagents optimized for cell signaling, metabolism, and membrane biology research.

Structurally, Sphingomyelin phosphodiesterase 3 contains an N-terminal hydrophobic domain for membrane association and a C-terminal catalytic domain requiring Mg2+ for activity. This antibody enables analysis of SMPD3i 1/2s role in ceramide-mediated signal transduction and cellular stress responses.

UniProt: Q9NY59

Pathways: Hormone Transport