JAK3 Antikörper (pTyr980, pTyr981) (HRP)

Produktnummer ABIN742892

Der Kaninchen Polyklonal Anti-JAK3-Antikörper wurde für ELISA validiert. Er ist geeignet, JAK3 in Proben von Human zu detektieren.

Kurzübersicht für JAK3 Antikörper (pTyr980, pTyr981) (HRP) (ABIN742892)

Target: JAK3 (Janus Kinase 3 (JAK3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAK3 Antikörper ist konjugiert mit HRP

Applikation: ELISA

Produktdetails anti-JAK3 Antikörper (HRP)

Bindungsspezifität: pTyr980, pTyr981

Spezifität: These phosphorylation sites are homologous to Tyr976 + Tyr977 in Mouse and Rat.

Kreuzreaktivität: Rind (Kuh), Pferd, Human, Maus, Schwein, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Jak3 around the phosphorylation site of Tyr980/981

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % Gentamicin.

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4 °C

Haltbarkeit: 12 months

Details zu JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Andere Bezeichnung: Jak3 +

Hintergrund:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 Molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Subcellular location: Cytoplasm

Synonyms: JAKL, LJAK, JAK-3, L-JAK, JAK3_HUMAN, Tyrosine-protein kinase JAK3, Janus kinase 3, Leukocyte janus kinase, JAK3

Gen-ID: 3718

UniProt: P52333

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response