PIK3CD Antikörper (AA 520-720)

Produktnummer ABIN7865035

Der Maus Monoklonal anti-PIK3CD Antikörper wird verwendet zum Nachweis von PIK3CD in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für PIK3CD Antikörper (AA 520-720) (ABIN7865035)

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PIK3CD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: PIK3CD-4639

Produktdetails anti-PIK3CD Antikörper

Bindungsspezifität: AA 520-720

Verwendungszweck: PIK3CD Antibody

Spezifität: Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85a and p85b), each possessing one SH3 and two SH2 domains. Various p110 forms have been identified. p110a and p110b interact with p85a, and p110a has also been shown to interact with p85b in vitro. It has been shown to bind p85a and b, but it apparently does not phosphorylate these subunits. p110d has the capacity to autophosphorylate and results in the nearly complete inactivation of the lipid kinase activity. Interestingly, p110g does not interact with the p85 subunits and has been shown to be activated by a and bg heterotrimeric G proteins. Two p110d isoforms have been identified and are widely expressed. Isoform 1 is expressed predominantly in leukocytes while isoform 2 is expressed in normal thymus, lung and spleen tissues.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human PIK3CD protein (around aa 520-720) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu PIK3CD

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Andere Bezeichnung: PIK3CD

Hintergrund:

5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, APDS, GRB1, IMD14, p110delta, p110dp85a, p85-ALPHA, Phosphoinositide 3 kinase B, Phosphoinositide 3 kinase C, Phosphoinositide 3 kinase catalytic delta polypeptide, Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta, PI3K-delta, PIK3R1, PtdIns 3 kinase p110, PtdIns 3 kinase subunit p110 delta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta

lymph node or tonsil|THP1 cells. Human spleen

Molekulargewicht: 119/33kDa

Gen-ID: 5293, 518451

UniProt: O00329

Pathways: BCR Signaling, Warburg Effekt