Rekombinanter MCM5 Antikörper (AA 500-734)

Produktnummer ABIN7865528

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch MCM5 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter MCM5 Antikörper (AA 500-734) (ABIN7865528)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MCM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: MCM5-13320R

Produktdetails anti-MCM5 Antikörper

Bindungsspezifität: AA 500-734

Expressionssystem: CHO Cells

Verwendungszweck: Recombinant MCM5 (Transcription Factor) Antibody

Spezifität: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double- stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-≥-induced Stat1± transcription activation.

Aufreinigung: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa500-734) of human MCM5 protein (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Andere Bezeichnung: MCM5

Hintergrund:

CDC46, MGORS8, P1-CDC46

Raji, Ramos or A-431 cells. Human tonsil, spleen or lymph node.

Molekulargewicht: 90kDa

Gen-ID: 4174

UniProt: P33992

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA