Rekombinanter ZAP70 Antikörper (AA 247-382)
Kurzübersicht für Rekombinanter ZAP70 Antikörper (AA 247-382) (ABIN7826139)
Target
Alle ZAP70 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 247-382
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Verwendungszweck
- ZAP-70 antibody (870R)
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Immunogen
- Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:75-1:150
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Kommentare
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Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.
Product Description/Background: ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.
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Protokoll
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Haltbarkeit
- 24 months
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Andere Bezeichnung
- ZAP-70
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Hintergrund
- Gene Alias: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa
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UniProt
- P43403
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Pathways
- T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
Target
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