GDNF Antikörper (AA 121-211)

Produktnummer ABIN736536

Dieses Anti-GDNF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GDNF in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Rind (Kuh) und Kaninchen. Unabhängig validiert für die Verwendung in Immunofluorescence. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für GDNF Antikörper (AA 121-211) (ABIN736536)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-GDNF Antikörper

Bindungsspezifität: AA 121-211

Kreuzreaktivität: Rind (Kuh), Human, Maus, Kaninchen, Ratte

Homologie: Dog,Cow,Pig,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GDNF

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #102003 (Immunofluorescence)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #102003

Datum: 12.02.2018

Antigen: GDNF

Chargennummer: 9F01M8

Validierte Anwendung: Immunofluorescence

Positivkontrolle: mouse hippocampus and putamen

Negativkontrolle: no primary control

Bewertung:

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Validierungsbilder

IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal horn of the spinal cord (B), and the hippocampus CA1 (C) and Putamen (D).

Protokoll

Primärantikörper: ABIN736536

Sekundärantikörper: anti-rabbit AF488 conjugated antibody (Life Technologies)

Full Protocol:

• Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
• Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
• Wash spinal cord and brain blocks several times with PBS.
• Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
• Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
• Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
• Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
• Wash sections 4x for 5min with 0.01M PBS.
• Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
• Wash sections 4x for 5min with 0.01M PBS.
• Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).

Anmerkungen:

• Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

• Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GDNF Antikörper (ABIN736536)

Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, (2014) (PubMed).

Details zu GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: GDNF

Hintergrund:

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

Background: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Gen-ID: 2668

UniProt: P39905

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration