PKM2 Antikörper

Produktnummer ABIN7674413

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PKM2 in WB, IHC, ELISA, IF und IP. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für PKM2 Antikörper (ABIN7674413)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-PKM2 Antikörper

Verwendungszweck: PKM2-specific antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: PKM2 pyruvate kinase, muscle

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:4000, IP: 1:200-1:1000, IHC: 1:100-1:400, IF: 1:10-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: PKM2

Hintergrund:

Synonyms: Pyruvate kinase PKM|Cytosolic thyroid hormone-binding protein (CTHBP)|Opa-interacting protein 3 (OIP-3)|Pyruvate kinase 2/3|Pyruvate kinase muscle isozyme|Threonine-protein kinase PKM2|Thyroid hormone-binding protein 1 (THBP1)|Tumor M2-PK|Tyrosine-protein kinase PKM2|p58|PKM|OIP3|PK2|PK3|PKM2

Background: PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1, however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms.The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.

Molekulargewicht: 58 kDa

UniProt: P14618

Pathways: Warburg Effekt