Leptin Antikörper

Produktnummer ABIN7670677

Dieses Anti-Leptin-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Leptin in ELISA (Capture). Geeignet für Human.

Kurzübersicht für Leptin Antikörper (ABIN7670677)

Target: Leptin (LEP)

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: ELISA (Capture)

Klon: 10G8

Produktdetails anti-Leptin Antikörper

Verwendungszweck: Leptin/OB Monoclonal Antibody(Capture)

Aufreinigung: Protein A/G Purification

Immunogen: Recombinant Human Leptin/OB protein expressed by E.coli

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA Capture 2-8 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Konservierungsmittel: Without preservative

Handhabung: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Haltbarkeit: 12 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin/OB

Hintergrund: LEP,Leptin,Obese protein,Obesity factor,OB,OBS,Key player in the regulation of energy balance and body weight control. Once released into the circulation,has central and peripheral effects by binding lEPR,found in many tissues,which results in the activation of several major signaling pathways.In the hypothalamus,acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides,also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery,increases basal metabolism,influences reproductive function,regulates pancreatic beta-cell function and insulin secretion,is pro-angiogenic for endothelial cell and affects innate and adaptive immunity.In the arcuate nucleus of the hypothalamus,activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides.In addition to its known satiety inducing effect,has a modulatory role in nutrient absorption. In the intestine,reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38,PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption.Acts as a growth factor on certain tissues,through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1,via JAK2-STAT3 pathway,or VEGFA,via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression.Pro-angiogenic,has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs).In innate immunity,modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells.

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process