Rekombinanter Tyrosinase-Related Protein 1 Antikörper (AA 257-377) (CF®594)

Produktnummer ABIN7661537

Der Kaninchen Monoklonal Anti-Tyrosinase-Related Protein 1-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Tyrosinase-Related Protein 1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Tyrosinase-Related Protein 1 Antikörper (AA 257-377) (CF®594) (ABIN7661537)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Tyrosinase-Related Protein 1 Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TYRP1-2340R

Produktdetails anti-Tyrosinase-Related Protein 1 Antikörper (CF®594)

Bindungsspezifität: AA 257-377

Verwendungszweck: Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) (TYRP1/2340R), CF594 conjugate

Produktmerkmale: This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-Related Protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human TYRP1 protein (around aa 257-377)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30 cells. Human Skin or Melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Andere Bezeichnung: Tyrosinase Related Protein-1

Hintergrund:

Synonyms: 5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75, Tyrosinase-related protein 1 (TYRP1), TYRRP

Gene Symbol: TYRP1

Tissue Expression: Melanocytes

Molekulargewicht: 75 kDa

Gen-ID: 7306

UniProt: P17643