TRIM29 Antikörper (AA 1-200) (CF®740)

Produktnummer ABIN7661077

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TRIM29 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TRIM29 Antikörper (AA 1-200) (CF®740) (ABIN7661077)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRIM29 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: TRIM29-1042

Produktdetails anti-TRIM29 Antikörper (CF®740)

Bindungsspezifität: AA 1-200

Verwendungszweck: TRIM29(TRIM29/1042), CF740 conjugate

Produktmerkmale: This antibody recognizes a 66 kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90 % positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: A431 cells. Tonsil or Squamous cell carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Andere Bezeichnung: TRIM29

Hintergrund:

Synonyms: Ataxia telangiectasia group D complementing gene (ATDC), Tripartite motif-containing protein 29 (TRIM29),

Gene Symbol: TRIM29

Molekulargewicht: 66 kDa

Gen-ID: 23650

UniProt: Q14134

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus